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EMSA问题/可能答案
有关EMSA的各种问题及可能解决方案还没全部完成，遇到问题的请耐心看看，欢迎提意见。
| Methods | Problems /Solution | Major procedures | 微奥基因's products |
|---|---|---|---|
| Pre-EMSA | Well designing is a guarantee of success | Experimental design | Willing to check experimental designs for those using our services |
| EMSA samples and possible problems | Sample preparation |
Nuclear protein prep. Nuc. protein determ. Positive controls. Sample Prep. service |
|
| High quality probes are nescessary for EMSA | Probe preparation |
Ready biotin-probes Ready IR Fluo-probes Custom EMSA probes |
|
| Biotin EMSA | See how to make high quality gels | Casting gels |
Precasting gels Gel-making sol. Running buffers |
| ECL EMSA-related problems and their solution. |
Protein-probe reaction Running gels Transblots UV crosslink Biotin/Avidin/HRP reaction ECL Imaging |
Complete EMSA kits General EMSA kits EMSA reagents ECL substrates ECL imagers EMSA controls EMSA Services |
|
| IR Dye EMSA | |||
| See how to make high quality gels | Casting gels |
Precasting gels Gel-making sol. Running buffers |
|
| IR EMSA-related problems and their solution. |
Protein-probe Reaction Running gels Imaging gels |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services |
|
|
Gel-Stain EMSA |
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| See how to make high quality gels | Casting gels |
Precasting gels Gel-making sol. Running buffers |
|
| Gel-stain EMSA-related problems and their solution. |
Protein-probe Reaction Running gels Staining gels Imaging gels |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services |
|
|
Competitive EMSA |
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| See how to make high quality gels | Casting gels |
Precasting gels Gel-making sol. Running buffers |
|
| Competitive（竞争） EMSA-related problems and their solution. |
Protein-probe Reaction Running gels Staining gels Imaging gels |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services |
|
|
Supershift EMSA |
See how to make high quality gels | Casting gels |
Precasting gels Gel-making sol. Running buffers |
| Supershift （超迁移）EMSA-related problems and their solution. |
Protein-probe Reaction The rest is same as ECL or IR EMSA |
Ready probes Custom probes Complete EMSA kits General EMSA kits EMSA reagents ECL imagers EMSA controls EMSA Services |
|
| RNA EMSA | |||
| See how to make high quality gels | Casting gels |
Precasting gels Gel-making sol. Running buffers |
|
| RNA EMSA-related problems and their solution. |
Protein-probe Reaction The rest is same as ECL or IR EMSA |
Custom probes General EMSA kits EMSA reagents ECL imagers EMSA controls RNA EMSA Service |
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